ABSTRACT
Objective To investigate the inhibitory effect of Bletilla striata extracts(BSE)on bleomycin-induced pulmonary fibrosis(PF)in rats and study the chemical characteristics of the active component. Methods B. striata was extracted with 95%ethyl?alcohol to obtain the ethanol extract. The residue was extracted with water and precipitated with ethanol to obtain the B. striata polysac?charide(BSP). Sprague Dawley male rats were intratracheally administered of bleomycin(BLM,5 mg/kg)to induce PF. PF Rats were administered with ethanol BSE(300 mg/kg body mass)and BSP(300 mg/kg body mass)once daily for 28 d by gavage. On 14 d and 28 d, the lungs were harvested to observe the pathological and collagen deposition changes. The lung coefficient and the content of hydroxy?proline(HYP)were also determined. The phenol-sulfuric acid method was used to detect the total sugar content,the relative molecular mass was detected by high performance gel permeation chromatography(HPGPC)and the monosaccharide components were analyzed by high performance liquid chromatography(HPLC). Results Compared with PF model group,the histopathological changes in lung tissue treated with BSP group were obviously improved ,the level of HYP in lung tissues and the lung coefficient were significantly decreased (P<0.05,P<0.01). But the group treated with ethanol BSE had no significant improvement. The content of BSP was 81.41%,and the number average molecular weight(Mn)of BSP was about 2.23 × 105. The BSP was mainly composed of glucose and mannose. Conclusion HYP could significantly decrease lung HYB and collagen deposition of PF rats,and has the potiential for inhi?bition of PF.
ABSTRACT
Objective To investigate the effect of Euryale ferox seeds ethanol extract on renal funtion of diabetic nephropathy (DN) induced by strepotozotocin (STZ) in rats and the antioxidant ability of ethanol extract,water extract supernatant and precipitation. Methods Ethanol extract of Euryale ferox seeds were prepared by ethanol extraction and followed by evaporation and freeze-dry. The seeds residue was further extracted with water and precipitated with ethanol to obtain water extract supernatant and precipitation. Sprague Dawley male rats were given intraperitoneal injection of STZ at a dose of 60 mg/kg to induce DN. STZ-induced DN rats were administrated by gavage with different dose of Euryale ferox seeds ethanol extract(75, 150, 300 and 600 mg/kg body weight) once daily for 12 weeks. 24 h urine was collected for examining urinary protein and microalbumin every four weeks. Blood serum was also collected for examining of serum creatinine (Cr) and blood urea nitrogen (BUN) level after rats sacrificed at the end of 12 weeks after administration, and the pathological changes of kidney were observed. Antioxidant capacity of the three extracts were determined by Fe3+ reduction, and radical scavenging rate to 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazl(DPPH), O2-and OH-free radicals. Results Compared with model group, the content of 24 h urine protein and urine microalbumin of DN rats were reduced in all Euryale ferox seeds ethanol extract administration groups, and 24 h proteinuria were significantly reduced in the group of 150 and 600 mg/kg administration(P<0.05);BUN level was also reduced in all dose of Euayale ferox seeds ethanol extract administration, and more remarkably reduced in the group of 300mg/kg (P<0.01). 12 weeks after administration, kidney damage amelioration was observed in pathological sections of rats in Euryale ferox seeds ethanol extracts 300 and 600 mg/kg groups. Meanwhile, Euryale ferox seeds ethanol extract showed a certain antioxidant abilities in reducing of Fe3+ and scavenging DPPH, O2- and OH- at the concentrations of 0.6, 0.8 and 1.0 mg/ml. Conclusion Euryale ferox seeds ethanol extract could decrease proteinuria of STZ-induced DN rats,this may be associated with its antioxidant capacity.
ABSTRACT
pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.